Hepatoprotective and antioxidant activities of the separate and combined administration of methanolic extract of Adasonia digitata and Cochorous olitorious leaves on Rats
Keywords:
Adasonia digitata, Cochorous olitorious, Histopathology, Antioxidant and HepatoprotectiveAbstract
The hepatoprotective and antioxidant activities of the separate and combined administration of methanolic extract of combine administration of Adasonia digitata and Cochorous olitorious leaves on Rats was investigated. During which Forty –two rats were grouped into 7 groups of 6 animals each. Group A rats received 2mL of distilled water, while groups B, C,D,E,F and G received equal volume corresponding to 500 and 1000 mg/kg body weight of the extract, respectively, for 14 days. The rats were sacrificed 24 h after 14days administration. The qualitative and quantitative phytochemical constituent of methanolic extracts of Adasonia digitata and Cochorous olitorious leaves was screened and was said to contain Saponins (A.dgitata:16.58 and C.olitorous:22.17), alkaloids (A.dgitata:81.56and C.olitorous:68.65), tannins (A.dgitata:311.98and C.olitorous:287.07), phenolics (A.dgitata:170.90and C. olitorous: 330.07), flavonoids (A.dgitata:25.38and C.olitorous:157.38). The level of liver serum alanine amino transferases (ALT), aspartate amino transferase (AST) and alkaline phosphatase (ALP) shows no significant (P<0.05) differences from the control group. Similar result was also obtained from the liver histological profile showing no visible damage when compared with the control group. There was no significance (P<0.05) differences between the heamatological profile, protein level, total bilirubin in the administered group when compared with the control group. The level of Superoxide dismutase, Catalase, Glutathione-S-transferase, Glutathione peroxidase enzymes were significantly (P<0.05) increase in the administered group when compared with the control group. It is therefore logical to conclude that, the plant may be explored as oral remedy in the treatments of free radical induced and inducing diseases.