Isolation, Characterization and Biological activities of ethyl acetate extract of Heliotropium indicum (whole plant)

Abstract

Fresh whole plant of Heliotropium indicum was collected, air dried, pulverized and successively extracted with n-hexane, ethyl acetate and ethanol to obtain n-hexane, ethyl acetate and ethanol extracts respectively. The phytochemical screening of the extracts revealed the presence of glycosides, cardiac glycosides, tannins, terpenoids and polyphenols in all the three extracts. Alkaloids, saponins, flavonoids and steroids were present in ethyl acetate and ethanol extracts. The antimicrobial sensitivity tests of the crude extracts on three gram positive bacteria, five gram negative bacteria and five fungi showed significant inhibition zones against most of the tested organisms. The isolated compound from the ethyl acetate extract was characterized with Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis and data matching with standard in literature.